用于研究人类精子趋化性的新型模型。
Novel model for study of human sperm chemotaxis.
作者信息
Villanueva-Díaz C, Arias-Martínez J, Bustos-López H, Vadillo-Ortega F
机构信息
Instituto Nacional de Perinatología, México City, México.
出版信息
Fertil Steril. 1992 Aug;58(2):392-5. doi: 10.1016/s0015-0282(16)55209-1.
OBJECTIVE
To develop and test an adequate method for the study of sperm chemotaxis.
DESIGN
A model based on a double-chamber design and a matrix of 0.5% hyaluronic acid was used to test the response of human sperm to chemotactic signals from follicular fluid (FF).
RESULTS
A plateau of migration was reached at 10 minutes of incubation; under this condition, 70% of sperm migrated toward chambers with FF, less than 20% to the opposite side, and the rest remained at the site of application. Intra-assay and interassay coefficients of variation using pools of FFs were 17.2% and 23%, respectively.
CONCLUSION
These data confirm previous findings and support the use of this novel model for the study of chemotaxis on human gametes.
目的
开发并测试一种适用于研究精子趋化性的方法。
设计
使用基于双室设计和0.5%透明质酸基质的模型来测试人类精子对卵泡液（FF）趋化信号的反应。
结果
孵育10分钟时达到迁移平台期；在此条件下，70%的精子向含有卵泡液的腔室迁移，不到20%向另一侧迁移，其余精子留在应用部位。使用卵泡液池的批内和批间变异系数分别为17.2%和23%。
结论
这些数据证实了先前的发现，并支持使用这种新模型来研究人类配子的趋化性。
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