人精子对卵泡液的趋化反应能力在获能后增强，但在精子异常的精液中则受损。

Chemotactic responsiveness of human spermatozoa to follicular fluid is enhanced by capacitation but is impaired in dyspermic semen.

作者信息

Tacconis P, Revelli A, Massobrio M, Battista La Sala G, Tesarik J

机构信息

Department of Obstetrical and Gynecological Sciences, St. Anna Hospital, University of Torino, Via Ventimiglia 3, 10126 Torino, Italy.

出版信息

J Assist Reprod Genet. 2001 Jan;18(1):36-44. doi: 10.1023/a:1026402830232.

DOI:10.1023/a:1026402830232

PMID:11292995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455816/

Abstract

PURPOSE

Our purpose was to study the chemotactic responsiveness of human spermatozoa from normal and pathological semen samples to follicular fluid (FF), as well as the effect exerted by capacitation on sperm chemotaxis.

METHODS

The chemotactic responsiveness of human spermatozoa to FF was tested by an accumulation assay chamber in which they were allowed to migrate through a microporous membrane and accumulate in a compartment filled with FF or control medium. The percentage of cells with hyperactivated motility among migrated sperm was objectively assessed by CASA and its relationship to the accumulation rate was studied. Single FFs were tested with single normospermic or dyspermic semen samples; the same FF was tested with different semen specimens. The influence of capacitation onto the chemotactic responsiveness to FF was investigated by comparing the accumulation rate of spermatozoa from normal and pathological samples after incubation under capacitating conditions for 1 or 6 hr.

RESULTS

Some FFs ("active" FFs) effectively attracted human spermatozoa from normospermic samples up to a dilution factor of 1:500 (v:v) with control medium. A wide range of responses was observed when the same FF was tested with different normal semen samples. A longer time under capacitating conditions increased both the chemotactic responsiveness of normal semen and its ability to undergo the acrosome reaction (AR) in response to A23187. Pathological semen had an impaired chemotactic responsiveness to "active" FF that was not enhanced by increasing the time spent under capacitating conditions. Dyspermic semen was also less responsive to A23187, a finding suggesting incomplete capacitation.

CONCLUSIONS

Chemotactic responsiveness to FF is acquired in parallel to or as part of the capacitation process. Dyspermic semen samples have an impaired capacity to achieve both capacitation and chemotactic responsiveness to follicular factors.

摘要

目的

我们的目的是研究来自正常和病理精液样本的人类精子对卵泡液（FF）的趋化反应性，以及获能对精子趋化性的影响。

方法

通过聚集试验室测试人类精子对FF的趋化反应性，在该试验室中，精子被允许穿过微孔膜并聚集在充满FF或对照培养基的隔室中。通过计算机辅助精子分析（CASA）客观评估迁移精子中具有超激活运动能力的细胞百分比，并研究其与聚集率的关系。单个FF与单个正常精子或异常精子精液样本进行测试；相同的FF与不同的精液标本进行测试。通过比较在获能条件下孵育1或6小时后正常和病理样本中精子的聚集率，研究获能对FF趋化反应性的影响。

结果

一些FF（“活性”FF）能有效吸引来自正常精子样本的人类精子，稀释至1:500（体积比）时仍能与对照培养基相区分。用相同的FF测试不同的正常精液样本时，观察到广泛的反应范围。在获能条件下更长时间孵育，既增加了正常精液的趋化反应性，也增加了其对A23187作出顶体反应（AR）的能力。病理精液对“活性”FF的趋化反应性受损，增加获能条件下的孵育时间并不能增强这种反应性。异常精子精液对A23187的反应也较弱，这一发现提示获能不完全。