Wang Mei-hui, Harshman Lawrence G, Nuzhdin Sergey V
School of Biological Sciences, University of Nebraska, Lincoln, USA.
Obes Res. 2005 Nov;13(11):1891-7. doi: 10.1038/oby.2005.232.
Recombinant inbred lines derived from a natural population were used to investigate natural genetic variation for lipid abundance, protein abundance, and weight of Drosophila melanogaster. Females were heavier and contained more lipid and soluble protein than males. Lipid and protein abundance were genetically correlated with female weight, but male weight was not correlated with lipid or protein. Lipid and protein abundance were genetically correlated in males, but not in females. Quantitative trait loci (QTLs) for weight and protein abundance were predominantly on the X chromosome, whereas QTLs for lipid abundance were found on the second and third chromosomes. QTLs for lipid proportion (lipid abundance normalized by weight or protein abundance) were present on all chromosomes; a lipid proportion QTL on the third chromosome correlated with a QTL for starvation resistance observed in a previous study using the same set of recombinant inbred lines, suggesting that it might underlie both traits. Candidate genes are discussed in relationship to lipid abundance, lipid proportion, and starvation resistance.
源自自然种群的重组自交系被用于研究黑腹果蝇脂质丰度、蛋白质丰度和体重的自然遗传变异。雌性比雄性更重，且含有更多的脂质和可溶性蛋白质。脂质和蛋白质丰度与雌性体重存在遗传相关性，但雄性体重与脂质或蛋白质无关。脂质和蛋白质丰度在雄性中存在遗传相关性，但在雌性中不存在。体重和蛋白质丰度的数量性状基因座（QTL）主要位于X染色体上，而脂质丰度的QTL则位于第二和第三条染色体上。所有染色体上均存在脂质比例（通过体重或蛋白质丰度标准化的脂质丰度）的QTL；第三条染色体上的一个脂质比例QTL与先前使用同一组重组自交系进行的一项研究中观察到的抗饥饿QTL相关，这表明它可能是这两个性状的基础。文中讨论了与脂质丰度、脂质比例和抗饥饿性相关的候选基因。
