Solomon J E, Paul M R
Condensed Matter Physics, California Institute of Technology, Pasadena, California, USA.
Biophys J. 2006 Mar 1;90(5):1842-52. doi: 10.1529/biophysj.105.067835. Epub 2005 Dec 9.
This article presents a number of kinetic analyses related to binding processes relevant to capture of target analyte species in nanoscale cantilever-type devices designed to detect small concentrations of biomolecules. The overall analyte capture efficiency is a crucial measure of the ultimate sensitivity of such devices, and a detailed kinetic analysis tells us how rapidly such measurements may be made. We have analyzed the capture kinetics under a variety of conditions, including the possibility of so-called surface-enhanced ligand capture. One of the modalities studied requires ligand capture through a cross-linking mechanism, and it was found that this mode may provide a robust and sensitive approach to biomolecular detection. For the two modalities studied, we find that detection of specific biomolecules down to concentration levels of 1 nM or less appear to be quite feasible for the device configurations studied.
本文介绍了一些与结合过程相关的动力学分析，这些结合过程与在设计用于检测低浓度生物分子的纳米级悬臂式设备中捕获目标分析物物种有关。整体分析物捕获效率是此类设备最终灵敏度的关键指标，详细的动力学分析告诉我们进行此类测量的速度有多快。我们分析了多种条件下的捕获动力学，包括所谓的表面增强配体捕获的可能性。所研究的一种模式需要通过交联机制进行配体捕获，并且发现这种模式可能为生物分子检测提供一种强大而灵敏的方法。对于所研究的两种模式，我们发现对于所研究的设备配置，检测低至1 nM或更低浓度水平的特定生物分子似乎是相当可行的。
