在用基因工程改造后能产生卵清蛋白的大肠杆菌定殖的大鼠中诱导产生针对卵清蛋白的IgE抗体和T细胞反应性。
Induction of IgE antibodies and T-cell reactivity to ovalbumin in rats colonized with Escherichia coli genetically manipulated to produce ovalbumin.
作者信息
Dahlman A, Ahlstedt S, Hanson L A, Telemo E, Wold A E, Dahlgren U I
机构信息
Department of Clinical Immunology, University of Göteborg, Sweden.
出版信息
Immunology. 1992 Jun;76(2):225-8.
The immune response to ovalbumin (OA) and the bacterial antigens, lipopolysaccharide (LPS) and fimbriae were studied in conventional rats colonized from birth with an Escherichia coli strain producing OA. The colonized rats had developed IgE antibodies against OA, but not against the fimbrial or the LPS antigens from the E. coli at 2 months of age. At this time all rats were primed with OA given intracutaneously in Freund's complete adjuvant. Two weeks later the colonized rats showed a 35% greater delayed-type hypersensitivity (DTH) reaction to OA, measured as ear swelling, than the controls. Thus bacteria carrying antigens resembling potential allergens might aggravate, or participate in the induction of allergic symptoms. In addition such bacteria could be efficient vaccine vectors in protection against parasites. The study illustrates the importance of the mode of antigen presentation for the subsequent immune response.
在出生后即定殖有一株产生卵清蛋白（OA）的大肠杆菌的普通大鼠中，研究了对卵清蛋白（OA）以及细菌抗原脂多糖（LPS）和菌毛的免疫反应。定殖的大鼠在2月龄时已产生针对OA的IgE抗体，但未产生针对大肠杆菌菌毛或LPS抗原的IgE抗体。此时，所有大鼠均用弗氏完全佐剂皮内注射OA进行致敏。两周后，定殖大鼠对OA的迟发型超敏反应（DTH，以耳肿胀衡量）比对照大鼠高35%。因此，携带类似于潜在过敏原的抗原的细菌可能会加重或参与过敏症状的诱导。此外，这类细菌可能是预防寄生虫的有效疫苗载体。该研究说明了抗原呈递方式对后续免疫反应的重要性。
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