Salgado J, Gilabert A, Franch A, Castell M, Castellote C, Queralt J
Departament de Ciències Fisiològiques Humanes i de la Nutrició, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Allergol Immunopathol (Madr). 1989 Sep-Oct;17(5):237-40.
Specific and total IgG and IgE serum levels were measured in Sprague-Dawley rats after immunization with ovalbumin (OA). OA was administered in an aqueous solution, suspended in alum or in Complete Freund's Adjuvant (CFA) with or without simultaneous administration of Bordetella pertussis (Bp). Control groups received only saline, alum or CFA. Specific IgG, total IgG and total IgE were determined by ELISA methods. Specific IgE was biologically evaluated. The highest specific IgG and IgE antibody responses were obtained with OA suspended in alum, while Bp failed to potentiate this response. The low specific response seen with OA suspended in CFA was potentiated when Bp was simultaneously dosed. Total IgE levels were increased in those groups receiving Bp. The contribution of specific IgG or IgE response to the total IgG or IgE levels was not distinguished with this experimental procedure. It can be concluded that immunization with OA suspended in alum induces appreciable increases in specific IgG and IgE antibodies without significantly affecting total IgG and IgE levels.
用卵清蛋白（OA）免疫斯普拉格-道利大鼠后，检测其血清特异性IgG和IgE以及总IgG和IgE水平。OA以水溶液形式给药，悬浮于明矾中或与或不与百日咳博德特氏菌（Bp）同时给药的完全弗氏佐剂（CFA）中。对照组仅接受生理盐水、明矾或CFA。通过ELISA方法测定特异性IgG、总IgG和总IgE。对特异性IgE进行生物学评估。悬浮于明矾中的OA产生了最高的特异性IgG和IgE抗体反应，而Bp未能增强这种反应。当同时给予Bp时，悬浮于CFA中的OA所产生的低特异性反应得到增强。接受Bp的组中总IgE水平升高。该实验程序未区分特异性IgG或IgE反应对总IgG或IgE水平的贡献。可以得出结论，用悬浮于明矾中的OA免疫可诱导特异性IgG和IgE抗体显著增加，而不会显著影响总IgG和IgE水平。
