Zhou X H, Kawakami H, Hirano H
Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan.
Histochem J. 1992 Jun;24(6):354-60. doi: 10.1007/BF01046167.
Changes in the lectin binding of mouse Leydig cells during fetal and postnatal development were examined by light- and electron-microscopy using eight different biotinylated lectins (ConA, WGA, RCA-I, UEA-I, GS-I, PNA, SBA and GS-II). At the light-microscopic level, ConA, WGA, RCA-I, UEA-I and GS-I showed the same binding pattern in which all five lectins bound to the plasma membrane and cytoplasm of Leydig cells from the 13th day post coitum (p.c.) to the 8th postnatal week. PNA, SBA and GS-II reactions were positive in the plasma membrane and cytoplasm of Leydig cells from the 13th day p.c. to 15th day post partum (p.p.) but disappeared completely by day 20. At the electron-microscopic level, gold particles representing the GS-I or GS-II binding sites were distributed primarily along the cell surface membrane, including that of microvilli, as well as in the cytoplasm. These results indicate that certain glycoconjugates bearing D-galactose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine residues are expressed on the cell surface and in the cytoplasm of Leydig cells during the period from the 13th day p.c. to around the 20th day p.p. The results suggest that these glycoconjugates might play some role in modulating hormone-receptor interaction in the Leydig cells before the 20th day. Furthermore, these results may indicate that sugar residues expressed on the cell surface and in the cytoplasm of Leydig cells are different from those in the fetal-neonatal and adult phases.
利用八种不同的生物素化凝集素（伴刀豆球蛋白A、小麦胚凝集素、蓖麻凝集素-I、荆豆凝集素-I、大豆凝集素-I、花生凝集素、大豆凝集素和大豆凝集素-II），通过光学显微镜和电子显微镜检查了小鼠睾丸间质细胞在胎儿期和出生后发育过程中凝集素结合的变化。在光学显微镜水平上，伴刀豆球蛋白A、小麦胚凝集素、蓖麻凝集素-I、荆豆凝集素-I和大豆凝集素-I显示出相同的结合模式，即从交配后第13天（p.c.）到出生后第8周，所有这五种凝集素都与睾丸间质细胞的质膜和细胞质结合。花生凝集素、大豆凝集素和大豆凝集素-II反应在交配后第13天到产后第15天（p.p.）的睾丸间质细胞质膜和细胞质中呈阳性，但在第20天时完全消失。在电子显微镜水平上，代表大豆凝集素-I或大豆凝集素-II结合位点的金颗粒主要分布在细胞表面膜，包括微绒毛的膜，以及细胞质中。这些结果表明，在交配后第13天到产后第20天左右的这段时间里，某些带有D-半乳糖、N-乙酰-D-半乳糖胺和N-乙酰-D-葡萄糖胺残基的糖缀合物在睾丸间质细胞的细胞表面和细胞质中表达。结果表明，这些糖缀合物可能在第20天之前调节睾丸间质细胞中的激素-受体相互作用中发挥某种作用。此外，这些结果可能表明，睾丸间质细胞的细胞表面和细胞质中表达的糖残基与胎儿-新生儿期和成年期的不同。
