Kawakami H, Hirano H
Histochemistry. 1984;80(5):415-20. doi: 10.1007/BF00495428.
Carbohydrate moieties on the surface of dissociated rat liver cells were examined electron microscopically using ferritin- or horseradish peroxidase (HRP)-conjugated lectins as probes. Rat liver was fixed by perfusion with 0.7% glutaraldehyde via the portal vein and dissociated into single cells with gentle homogenization. Concanavalin A (Con A), Ricinus communis agglutinin (RCA), and wheat germ agglutinin (WGA) bound almost evenly to the entire cell surface of hepatocytes as well as of endothelial cells. Ulex europaeus agglutinin I (UEA-I) and peanut agglutinin (PNA) revealed no binding to any region. Dolichos biflorus agglutinin (DBA) was found to bind exclusively to the sinusoidal surface of hepatocytes and to endothelial cell surfaces. Soybean agglutinin (SBA)-binding was restricted to the endothelial cell surfaces and part of the sinusoidal microvilli of hepatocytes. Regional differences in lectin-binding pattern were visualized between the sinusoidal and the lateral or bile-canalicular surfaces of the hepatocytes. A polarity may exist on the hepatocyte cell surfaces in terms of the distribution pattern of the carbohydrate moieties, especially those of N-acetylgalactosamine.
使用铁蛋白或辣根过氧化物酶（HRP）偶联的凝集素作为探针，通过电子显微镜检查解离的大鼠肝细胞表面的碳水化合物部分。通过门静脉用0.7%戊二醛灌注固定大鼠肝脏，并通过轻柔匀浆将其解离为单个细胞。刀豆球蛋白A（Con A）、蓖麻凝集素（RCA）和麦胚凝集素（WGA）几乎均匀地结合在肝细胞以及内皮细胞的整个细胞表面。欧洲荆豆凝集素I（UEA-I）和花生凝集素（PNA）未显示与任何区域结合。发现双花扁豆凝集素（DBA）仅与肝细胞的窦状表面和内皮细胞表面结合。大豆凝集素（SBA）的结合仅限于内皮细胞表面和肝细胞部分窦状微绒毛。在肝细胞的窦状表面与侧面或胆小管表面之间观察到凝集素结合模式的区域差异。就碳水化合物部分尤其是N-乙酰半乳糖胺的分布模式而言，肝细胞表面可能存在极性。
