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采用间接荧光抗体-膜过滤技术测定克利夫兰港中甲烷甲基单胞菌和三孢布拉氏霉菌的分布。

Distribution of Methylomonas methanica and Methylosinus trichosporium in Cleveland Harbor as Determined by an Indirect Fluorescent Antibody-Membrane Filter Technique.

机构信息

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210.

出版信息

Appl Environ Microbiol. 1978 Feb;35(2):422-30. doi: 10.1128/aem.35.2.422-430.1978.

DOI:10.1128/aem.35.2.422-430.1978
PMID:16345280
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242847/
Abstract

An indirect fluorescent antibody-membrane filter staining technique, which permitted the autecological study of Methylomonas methanica and Methylosinus trichosporium, was developed. This technique was used to assay the numbers of these two organisms in Cleveland Harbor. The concentrations of M. methanica and M. trichosporium were found to be inversely proportional to the sampling depth, with the highest cell counts observed in the sediments. M. methanica was observed at every sampling station, whereas M. trichosporium was found at only two of the stations.

摘要

建立了一种间接荧光抗体-膜过滤染色技术，该技术可用于对甲醇单胞菌和三孢布拉氏霉菌进行自生源研究。应用该技术对克利夫兰港中的这两种菌的数量进行了测定。结果表明，甲醇单胞菌和三孢布拉氏霉菌的浓度与采样深度呈反比，在沉积物中观察到的细胞数量最多。在每个采样点都观察到了甲醇单胞菌，而只在两个采样点发现了三孢布拉氏霉菌。

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Distribution of Methylomonas methanica and Methylosinus trichosporium in Cleveland Harbor as Determined by an Indirect Fluorescent Antibody-Membrane Filter Technique.采用间接荧光抗体-膜过滤技术测定克利夫兰港中甲烷甲基单胞菌和三孢布拉氏霉菌的分布。
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SOME ASPECTS OF METHANE OXIDATION.甲烷氧化的某些方面。
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