Munson T E, Schrade J P, Bisciello N B, Fantasia L D, Hartung W H, O'Connor J J
Appl Environ Microbiol. 1976 Apr;31(4):514-21. doi: 10.1128/aem.31.4.514-521.1976.
A prototype automated system using fluorescent antibody (FA) was evaluated for rapid detection of salmonellae in foods. Samples were enriched in selenite cystine and tetrathionate broths. After incubation, both were transferred into fresh selenite cystine for a 4-h "post-enrichment" to dilute possible background fluorescence from product. These cultures were then analyzed automatically, and results were compared with those obtained by the methods of the Association of Official Analytical Chemists (AOAC). Initially, 167 samples of milk powder, dried yeast, and imported frog legs were examined. The AOAC and automated FA methods correlated well with all samples but frog legs. Difficulty with the latter was caused by procedural and mechanical problems coupled with high numbers of competing microorganisms in post-enrichment cultures. Modification of procedure and partial redesign of equipment corrected these difficulties, and excellent correlation was obtained with another 116 frog leg samples. All 89 AOAC-confirmed positives were also detected by the automated FA method, and there were only 4% false FA positives. The system shows potential for screening products for salmonellae; however, all positives should be confirmed by manual biochemical and serological methods.
对一种使用荧光抗体（FA）的自动化原型系统进行了评估，以快速检测食品中的沙门氏菌。样品在亚硒酸盐胱氨酸肉汤和四硫磺酸盐肉汤中增菌。培养后，将两者转移至新鲜的亚硒酸盐胱氨酸肉汤中进行4小时的“增菌后培养”，以稀释产品可能产生的背景荧光。然后对这些培养物进行自动分析，并将结果与美国官方分析化学家协会（AOAC）的方法所获得的结果进行比较。最初，对167份奶粉、干酵母和进口蛙腿样品进行了检测。AOAC法和自动化FA法对除蛙腿以外的所有样品相关性良好。蛙腿检测出现困难是由于操作和机械问题，以及增菌后培养物中存在大量竞争性微生物。对操作程序的修改和设备的部分重新设计纠正了这些困难，并且对另外116份蛙腿样品获得了良好的相关性。所有89份经AOAC确认的阳性样品也通过自动化FA法检测到，并且FA法的假阳性率仅为4%。该系统显示了对产品进行沙门氏菌筛查的潜力；然而，所有阳性结果都应通过手动生化和血清学方法进行确认。
