Northern Regional Research Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1981 Aug;42(2):385-7. doi: 10.1128/aem.42.2.385-387.1981.
Inclusion bodies isolated from Spo Cr mutants of Bacillus thuringiensis were toxic for larvae of the European corn borer. Probit analysis revealed comparable toxicity between wild-type crystals (isolated from B. thuringiensis subsp. kurstaki) and crystals produced from two spore-free mutants of the same subspecies. Death of the larvae was due to starvation, presumably through delta-endotoxin-induced gut paralysis. Inclusion bodies pretreated with alpha-chymotrypsinogen were equally as toxic as native crystals for the insect larvae.
从苏云金芽孢杆菌 Spo Cr 突变体中分离出的包含体对欧洲玉米螟幼虫有毒。概率分析表明，野生型晶体（从苏云金芽孢杆菌亚种 kurstaki 中分离得到）和来自同一亚种的两个无孢子突变体产生的晶体之间具有相当的毒性。幼虫的死亡是由于饥饿，可能是由于δ-内毒素诱导的肠道瘫痪。用α-糜蛋白酶原预处理的包含体对昆虫幼虫的毒性与天然晶体相当。
