Agricultural Microbiology Section, Dairy and Food Science Department, University of Saskatchewan, Saskatoon, Canada, S7N 0W0.
Appl Environ Microbiol. 1982 Sep;44(3):747-50. doi: 10.1128/aem.44.3.747-750.1982.
A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and alpha-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of alpha-amylase activities in culture filtrates or column effluents.
一种新的淀粉水解检测方法，该方法不依赖于碘染色或使用与淀粉形成复合物的染料。在琼脂淀粉平板上，当以半对数标度绘出酵母菌落周围的净透明圈区域与同一酵母菌株在液体培养基中产生的酶水平之间的关系时，获得了线性关系。在来自三个不同来源的淀粉透明圈区域和α-淀粉酶水平之间也观察到了类似的关系。这些观察结果表明，该方法可用于突变体分离、菌株改良计划以及预测培养滤液或柱流出物中的α-淀粉酶活性。
