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用于检测α-淀粉酶和（1→4）-β-D-葡聚糖酶的新型显色底物。

New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase.

作者信息

McCleary B V

出版信息

Carbohydr Res. 1980 Nov 1;86(1):97-104. doi: 10.1016/s0008-6215(00)84584-x.

DOI:10.1016/s0008-6215(00)84584-x
PMID:6159974
Abstract

New chromogenic substrates have been developed for the quantitative assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase. These were prepared by chemically modifying amylose or cellulose before dyeing, to increase solubility. After dyeing, the substrates were either soluble or could be readily dispersed to form fine, gelatinous suspensions. Assays based on the use of these substrates are sensitive and highly specific for either alpha-amylase or (1 leads to 4)-beta-D-glucanase. The method of preparation can also be applied to obtain substrates for other endo-hydrolases.

摘要

已开发出用于定量测定α-淀粉酶和（1→4）-β-D-葡聚糖酶的新型显色底物。这些底物是通过在染色前对直链淀粉或纤维素进行化学修饰以提高溶解度而制备的。染色后，底物要么可溶，要么可容易地分散形成细小的凝胶状悬浮液。基于使用这些底物的测定方法对α-淀粉酶或（1→4）-β-D-葡聚糖酶具有敏感性和高度特异性。该制备方法也可用于获得其他内切水解酶的底物。

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