Gregory M R, Gregory W W, Bruns D E, Zakowski J J
J Clin Microbiol. 1983 Dec;18(6):1366-9. doi: 10.1128/jcm.18.6.1366-1369.1983.
Highly purified salivary alpha-amylase inhibited the growth of fresh isolates of Neisseria gonorrhoeae on GC agar base medium supplemented with 2% IsoVitaleX (BBL Microbiology Systems). Hydrolysis of starch in the medium by amylase resulted in a negative starch-iodine test. However, purified amylase did not inhibit gonococcal growth on agar plates that contained hemoglobin (chocolate agar). This effect was not caused by inhibition of amylase, since amylase activity was the same in the presence or absence of blood products. Moreover, survival of N. gonorrhoeae in buffered saline was not affected by amylase. These results suggest that amylase inhibited the growth of N. gonorrhoeae on GC agar base plates by hydrolyzing starch.
高度纯化的唾液α淀粉酶可抑制淋病奈瑟菌新鲜分离株在添加了2% IsoVitaleX（BBL微生物系统公司）的GC琼脂基础培养基上生长。淀粉酶对培养基中淀粉的水解导致淀粉-碘试验呈阴性。然而，纯化的淀粉酶对含有血红蛋白的琼脂平板（巧克力琼脂）上的淋球菌生长没有抑制作用。这种效应不是由淀粉酶抑制引起的，因为无论有无血液制品存在，淀粉酶活性都是相同的。此外，缓冲盐溶液中淋病奈瑟菌的存活不受淀粉酶影响。这些结果表明，淀粉酶通过水解淀粉抑制淋病奈瑟菌在GC琼脂基础平板上生长。
