Swiss Federal Institute of Technology, Zürich, and Federal Institute for Water Resources and Water Pollution Control, CH-8600 Dubendorf, Switzerland.
Appl Environ Microbiol. 1983 Apr;45(4):1411-4. doi: 10.1128/aem.45.4.1411-1414.1983.
A mixed methanogenic culture was highly enriched in a growth medium containing propionate as the sole organic carbon and energy source. With this culture, the pathways of propionate degradation were studied by use of C-radiotracers. Propionate was first metabolized to acetate, carbon dioxide, and hydrogen by nonmethanogenic organisms. Formate was not excreted. The carbon dioxide originated exclusively from the carboxyl group of propionate, whereas both [2-C]- and [3-C]propionate lead to the production of radioactive acetate. The methyl and carboxyl groups of the acetate produced were equally labeled, regardless of whether [2-C]- or [3-C]propionate was used. These observations suggest that in the culture, propionate was degraded through a randomizing pathway.
在含有丙酸作为唯一有机碳源和能源的生长培养基中，高度富集了一种混合产甲烷菌。利用该培养物，通过 C 放射性示踪剂研究了丙酸的降解途径。丙酸首先被非产甲烷菌代谢为乙酸盐、二氧化碳和氢气。没有排出甲酸盐。二氧化碳仅来源于丙酸的羧基，而 [2-C]-和 [3-C]丙酸都导致放射性乙酸盐的产生。所产生的乙酸盐的甲基和羧基基团标记程度相同，无论使用 [2-C]-还是 [3-C]丙酸。这些观察结果表明，在该培养物中，丙酸通过随机途径降解。
