Corporate Research Science Laboratories, Exxon Research and Engineering Company, Linden, New Jersey 07036.
Appl Environ Microbiol. 1983 Jul;46(1):171-7. doi: 10.1128/aem.46.1.171-177.1983.
Sixteen new cultures of propane-utilizing bacteria were isolated from lake water from Warinanco Park, Linden, N.J. and from lake and soil samples from Bayway Refinery, Linden, N.J. In addition, 19 known cultures obtained from culture collections were also found to be able to grow on propane as the sole carbon and energy source. In addition to their ability to oxidize n-alkanes, resting-cell suspensions of both new cultures and known cultures grown on propane oxidize short-chain alkenes to their corresponding 1,2-epoxides. Among the substrate alkenes, propylene was oxidized at the highest rate. In contrast to the case with methylotrophic bacteria, the product epoxides are further metabolized. Propane and other gaseous n-alkanes inhibit the epoxidation of propylene. The optimum conditions for in vivo epoxidation are described. Results from inhibition studies indicate that a propane monooxygenase system catalyzes both the epoxidation and hydroxylation reactions. Experiments with cell-free extracts show that both hydroxylation and epoxidation activities are located in the soluble fraction obtained after 80,000 x g centrifugation.
从新泽西州林登市瓦里南科公园的湖水以及从新泽西州林登市贝威炼油厂的湖水和土壤样本中分离到了 16 株利用丙烷的细菌新培养物。此外，从培养物收集获得的 19 种已知培养物也被发现能够以丙烷作为唯一的碳源和能源生长。除了能够氧化正烷烃外，生长在丙烷上的新培养物和已知培养物的静止细胞悬液还将短链烯烃氧化为相应的 1,2-环氧化物。在底物烯烃中，丙烯被氧化的速率最高。与甲基营养细菌的情况不同，产物环氧化物进一步被代谢。丙烷和其他气态正烷烃抑制丙烯的环氧化作用。本文描述了体内环氧化的最佳条件。抑制研究的结果表明，丙烷单加氧酶系统催化环氧化和羟化反应。用无细胞提取物进行的实验表明，羟化和环氧化活性都位于 80,000 x g 离心后获得的可溶性部分中。
