Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543.
Appl Environ Microbiol. 1983 Aug;46(2):491-8. doi: 10.1128/aem.46.2.491-498.1983.
A new method is described that uses the fluorochrome primulin and epifluorescence microscopy for the enumeration of heterotrophic and phototrophic nanoplankton (2 to 20 mum). Phototrophic microorganisms are distinguished from heterotrophs by the red autofluorescence of chlorophyll a. Separate filter sets are used which allow visualization of the primulin-stained nanoplankton without masking chlorophyll a fluorescence, thus allowing easy recognition of phototrophic cells. Comparison with existing epifluorescence techniques for counting heterotrophic and phototrophic nanoplankton shows that primulin provides more accurate counts of these populations than the fluorescein isothiocyanate or proflavine techniques. Accuracy is comparable to that with the acridine orange technique, but this method requires only one filter preparation for the enumeration of both phototrophic and heterotrophic populations.
描述了一种新的方法，该方法使用荧光染料原花青素和荧光显微镜来计数异养和光养纳米浮游生物（2 至 20 微米）。通过叶绿素 a 的红色自体荧光将光养微生物与异养生物区分开来。使用单独的滤光片设置，在不掩盖叶绿素荧光的情况下可以对原花青素染色的纳米浮游生物进行可视化，从而可以轻松识别光养细胞。与现有的用于计数异养和光养纳米浮游生物的荧光显微镜技术相比，原花青素比荧光素异硫氰酸酯或吖啶橙技术更能准确地计数这些群体。其准确性与吖啶橙技术相当，但该方法仅需一种滤光片准备即可对光养和异养种群进行计数。
