Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47906.
Appl Environ Microbiol. 1984 Apr;47(4):870-2. doi: 10.1128/aem.47.4.870-872.1984.
Seven strains of Microcyclus aquaticus were found to be capable of growth on methanol and hydrogen as energy sources. Carbon was incorporated as CO(2) via the Calvin cycle, as shown by the presence of ribulosebisphosphate carboxylase activity in methanol-grown cells and by the absence of key enzymes of the ribulose monophosphate and serine pathways. In addition, incoporation of [C]methanol into cells was diminished when cultures were incubated in gas atmospheres enriched with carbon dioxide.
发现七种水生小球藻能够以甲醇和氢气作为能源进行生长。通过甲醇生长细胞中存在核酮糖二磷酸羧化酶活性以及不存在核酮糖单磷酸和丝氨酸途径的关键酶,表明碳通过卡尔文循环被掺入为 CO(2)。此外,当培养物在富含二氧化碳的气体气氛中孵育时,[C]甲醇掺入细胞的量减少。