Loginova N V, Namsaraev B B, Trotsenko Iu A
Mikrobiologiia. 1978 Jan-Feb;47(1):168-70.
Metabolism of methanol was studied in Microcyclus aquaticus Z-238 in enzymological experiments. This facultative methylotroph was found to assimilate the carbon of methanol after oxidation to CO2 via the reductive pentose phosphate cycle. Dehydrogenases of methanol, formaldehyde and formate as well as ribulose-1,5-diphosphate carboxylase were induced during the methylotrophic growth of the bacterium. The serine pathway and the hexulose phosphate cycle were not involved in methanol metabolism owing to the absence of serine-glyoxylate aminotransferase and hexulose phosphate synthase, respectively.
在酶学实验中研究了水生微环菌Z-238中甲醇的代谢。发现这种兼性甲基营养菌通过还原性戊糖磷酸循环将甲醇氧化为二氧化碳后同化甲醇中的碳。在该细菌的甲基营养生长过程中,诱导产生了甲醇、甲醛和甲酸脱氢酶以及1,5-二磷酸核酮糖羧化酶。由于分别不存在丝氨酸-乙醛酸氨基转移酶和磷酸己酮糖合酶,丝氨酸途径和磷酸己酮糖循环不参与甲醇代谢。