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干燥介质对冷冻干燥乳酸菌残余水分含量和活力的影响。

Effect of drying medium on residual moisture content and viability of freeze-dried lactic Acid bacteria.

机构信息

Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, and Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina.

出版信息

Appl Environ Microbiol. 1985 Feb;49(2):413-5. doi: 10.1128/aem.49.2.413-415.1985.

DOI:10.1128/aem.49.2.413-415.1985
PMID:16346728
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238417/
Abstract

The effect of various substances on the relationship between residual moisture content and the viability of freeze-dried lactic acid bacteria has been studied. Compounds such as polymers, which display considerable ability in displacing water, showed no protective action during freeze-drying. Adonitol, on the other hand, produced the smallest change in water content at various times during drying and allowed the highest rate of survival.

摘要

研究了各种物质对冷冻干燥乳酸菌的残余水分含量与生存能力之间关系的影响。在冷冻干燥过程中,具有相当脱水能力的聚合物等化合物没有表现出保护作用。相反,甘露醇在干燥过程的不同时间内使水分含量的变化最小,并允许最高的存活率。

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The resistance to freeze-drying and to storage was determined as the cellular ability to recover its survival rate and acidification activity.将冻干和储存的抗性确定为细胞恢复其存活率和酸化活性的能力。
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本文引用的文献

1
The survival of bacteria during and after drying.细菌在干燥过程中及干燥后的存活情况。
J Hyg (Lond). 1951 Jun-Sep;49(2-3):220-46. doi: 10.1017/s0022172400044120.
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Factors affecting the viability of air-borne bacteria. III. The role of bonded water and protein structure in the death of air-borne cells.影响空气传播细菌生存能力的因素。III. 结合水和蛋白质结构在空气传播细菌死亡中的作用。
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Some factors affecting preservability of freeze-dried bacteria.一些影响冻干细菌保存性的因素。
J Hyg (Lond). 1961 Mar;59(1):77-91. doi: 10.1017/s0022172400038729.
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The effect of residual water on the survival of dried bacteria during storage.残留水分对干燥细菌储存期间存活率的影响。
J Gen Microbiol. 1958 Dec;19(3):624-33. doi: 10.1099/00221287-19-3-624.
5
Protective effect of adonitol on lactic acid bacteria subjected to freeze-drying.核糖醇对冻干乳酸菌的保护作用。
Appl Environ Microbiol. 1983 Jan;45(1):302-4. doi: 10.1128/aem.45.1.302-304.1983.
6
Comparative study of the efficiency of some additives in protecting lactic acid bacteria against freeze-drying.某些添加剂在保护乳酸菌免受冷冻干燥影响方面的效率比较研究。
Cryobiology. 1983 Oct;20(5):560-6. doi: 10.1016/0011-2240(83)90044-5.
7
Protein structure and freeze-drying: the effects of residual moisture and gases.蛋白质结构与冷冻干燥:残留水分和气体的影响
Cryobiology. 1971 Apr;8(2):145-52. doi: 10.1016/0011-2240(71)90022-8.
8
Effect of residual moisture content on the survival of freeze-dried bacteria during storage under various conditions.残余水分含量对冻干细菌在不同条件下储存期间存活率的影响。
Cryobiology. 1966 Mar-Apr;2(5):276-9. doi: 10.1016/s0011-2240(66)80135-9.
9
Proceedings: Some aspects of freezing and drying of microorganisms on the basis of cellular water.论文集:基于细胞水的微生物冷冻干燥的若干方面
Cryobiology. 1973 Nov;10(5):403-8. doi: 10.1016/0011-2240(73)90066-7.