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快速生长土壤微生物中质粒种属的通用鉴定方法。

General method for the identification of plasmid species in fast-growing soil microorganisms.

机构信息

Department of Genetics, Research School of Biological Sciences, Australian National University, Canberra, Australian Capital Territory 2601, Australia.

出版信息

Appl Environ Microbiol. 1985 Apr;49(4):1001-3. doi: 10.1128/aem.49.4.1001-1003.1985.

DOI:10.1128/aem.49.4.1001-1003.1985
PMID:16346763
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238487/
Abstract

Using a horizontal gel electrophoresis method, we demonstrated reproducibly the presence of indigenous plasmids in different Rhizobium, Agrobacterium, and Pseudomonas strains. The method yields a large amount of plasmid DNA and is sensitive in detecting megaplasmids with molecular weights higher than 5 x 10. In two Rhizobium meliloti strains, a megaplasmid other than the low-mobility plasmid already known was detected.

摘要

采用水平凝胶电泳方法，我们可重复性地证明了不同根瘤菌、农杆菌和假单胞菌菌株中天然质粒的存在。该方法可产生大量质粒 DNA，并且对于检测分子量高于 5×10 的大型质粒非常敏感。在两种苜蓿中华根瘤菌（Rhizobium meliloti）菌株中，除了已知的低迁移率质粒外，还检测到了另一种大型质粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/238487/1588c156efcb/aem00149-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/238487/1588c156efcb/aem00149-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/238487/1588c156efcb/aem00149-0278-a.jpg

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