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固氮螺菌菌株中质粒的可视化及固氮基因定位

Plasmid visualization and nif gene location in nitrogen-fixing Azospirillum strains.

作者信息

Plazinski J, Dart P J, Rolfe B G

出版信息

J Bacteriol. 1983 Sep;155(3):1429-33. doi: 10.1128/jb.155.3.1429-1433.1983.

DOI:10.1128/jb.155.3.1429-1433.1983
PMID:6309750
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217844/
Abstract

A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all eight strains investigated and not on endogenous plasmids.

摘要

一种改良的凝胶电泳技术提供了一种可重复的方法,用于检测和分离固氮螺菌属中分子量范围从12×10⁶到370×10⁶的质粒。用三叶草根瘤菌的nifHD区域进行分析表明,在所研究的所有8个菌株中,固氮螺菌的nif基因位于染色体上,而非内源性质粒上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/036c3f6a48cc/jbacter00244-0494-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/7f3d5ecf65e3/jbacter00244-0492-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/7dbdb34699dc/jbacter00244-0493-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/036c3f6a48cc/jbacter00244-0494-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/7f3d5ecf65e3/jbacter00244-0492-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/7dbdb34699dc/jbacter00244-0493-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77bd/217844/036c3f6a48cc/jbacter00244-0494-a.jpg

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本文引用的文献

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Plasmid. 1981 May;5(3):371-3. doi: 10.1016/0147-619x(81)90012-3.
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Megaplasmids in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum.植物相关细菌苜蓿中华根瘤菌和青枯雷尔氏菌中的巨型质粒。
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Rapid procedure for detection and isolation of large and small plasmids.用于检测和分离大小质粒的快速方法。
联合固氮菌和根瘤菌菌株导致结瘤抑制的相互作用。
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General method for the identification of plasmid species in fast-growing soil microorganisms.快速生长土壤微生物中质粒种属的通用鉴定方法。
Appl Environ Microbiol. 1985 Apr;49(4):1001-3. doi: 10.1128/aem.49.4.1001-1003.1985.
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Molecular diversity of plasmids bearing genes that encode toluene and xylene metabolism in Pseudomonas strains isolated from different contaminated sites in Belarus.从白俄罗斯不同污染地点分离出的假单胞菌菌株中携带编码甲苯和二甲苯代谢基因的质粒的分子多样性。
Appl Environ Microbiol. 2000 Jul;66(7):2842-52. doi: 10.1128/AEM.66.7.2842-2852.2000.
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Genome structure of the genus Azospirillum.固氮螺菌属的基因组结构。
J Bacteriol. 2000 Jul;182(14):4113-6. doi: 10.1128/JB.182.14.4113-4116.2000.
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Sym plasmid genes of Rhizobium trifolii expressed in Lignobacter and Pseudomonas strains.三叶草根瘤菌的共生质粒基因在木质杆菌属和假单胞菌属菌株中表达。
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Ann Microbiol (Paris). 1981 Jan-Feb;132A(1):3-18.
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