Plazinski J, Dart P J, Rolfe B G
J Bacteriol. 1983 Sep;155(3):1429-33. doi: 10.1128/jb.155.3.1429-1433.1983.
A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all eight strains investigated and not on endogenous plasmids.
一种改良的凝胶电泳技术提供了一种可重复的方法,用于检测和分离固氮螺菌属中分子量范围从12×10⁶到370×10⁶的质粒。用三叶草根瘤菌的nifHD区域进行分析表明,在所研究的所有8个菌株中,固氮螺菌的nif基因位于染色体上,而非内源性质粒上。