Fliermans C B, Bohlool B B, Schmidt E L
Appl Microbiol. 1974 Jan;27(1):124-9. doi: 10.1128/am.27.1.124-129.1974.
Fluorescent antibodies (FA) prepared for Nitrobacter agilis and N. winogradskyi were highly reactive in homologous staining. Low-level cross-reactions between the two species were removed by adsorption. All 15 pure-culture isolates of Nitrobacter tested reacted strongly with either N. agilis FA or N. winogradskyi FA. All pure-culture isolates from soils were determined to be N. winogradskyi; those from Mammoth Cave sediments and a cattle waste oxidation ditch were N. agilis. No cross-reaction was found in extensive tests that included five isolates of Nitrosomonas europaea and 668 heterotrophic aerobic and anaerobic bacteria isolated from soil, sewage, and cave sites. The FA preparations were used to detect Nitrobacter species in Mammoth Cave sediments, in a cattle waste oxidation ditch, and in surface waters and sediments of a river and to observe that N. winogradskyi can outgrow N. agilis in enrichment culture.
为活跃硝化杆菌和维氏硝化杆菌制备的荧光抗体（FA）在同源染色中具有高反应性。通过吸附去除了这两个物种之间的低水平交叉反应。所测试的15株硝化杆菌纯培养分离株与活跃硝化杆菌FA或维氏硝化杆菌FA都发生了强烈反应。所有从土壤中分离的纯培养物都被确定为维氏硝化杆菌；那些来自猛犸洞沉积物和一个牛粪氧化沟的是活跃硝化杆菌。在广泛的测试中未发现交叉反应，这些测试包括五株欧洲亚硝化单胞菌分离株以及从土壤、污水和洞穴地点分离的668株异养需氧和厌氧细菌。这些FA制剂被用于检测猛犸洞沉积物、牛粪氧化沟以及一条河流的地表水和沉积物中的硝化杆菌物种，并观察到在富集培养中维氏硝化杆菌能够超过活跃硝化杆菌生长。
