Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada K1A 0R6.
Appl Environ Microbiol. 1986 Aug;52(2):320-4. doi: 10.1128/aem.52.2.320-324.1986.
Yeasts able to grow on d-xylose were screened for the ability to hydrolyze xylan. Xylanase activity was found to be rare; a total of only 19 of more than 250 strains yielded a positive test result. The activity was localized largely in the genus Cryptococcus and in Pichia stipitis and its anamorph Candida shehatae. The ability to hydrolyze xylan was generally uncoupled from that to hydrolyze cellulose; only three of the xylan-positive strains also yielded a positive test for cellulolytic activity. Of the 19 xylanolytic strains, 2, P. stipitis CBS 5773 and CBS 5775, converted xylan into ethanol, with about 60% of a theoretical yield computed on the basis of the amount of d-xylose present originally that could be released by acid hydrolysis.
筛选能够以 d-木糖为碳源生长的酵母，以检测其木聚糖酶活性。结果发现木聚糖酶活性非常罕见；在 250 多个菌株中，只有 19 个菌株的检测结果为阳性。这些菌株的活性主要定位于隐球菌属、毕赤酵母及其无性型假丝酵母谢氏变种。木聚糖酶活性通常与纤维素酶活性无关；在 19 个木聚糖阳性菌株中，只有 3 个菌株对纤维素酶活性的检测结果也为阳性。在 19 个产木聚糖酶的菌株中，有 2 株，即毕赤酵母 CBS 5773 和 CBS 5775，可将木聚糖转化为乙醇，根据酸水解可释放的最初存在的 d-木糖量，理论产率约为 60%。
