质粒向运动发酵单胞菌抗生素敏感突变株的转移。
Transfer of Plasmids to an Antibiotic-Sensitive Mutant of Zymomonas mobilis.
机构信息
Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
出版信息
Appl Environ Microbiol. 1986 Aug;52(2):366-70. doi: 10.1128/aem.52.2.366-370.1986.
Wild-type strains of Zymomonas mobilis exhibit multiple antibiotic resistance and thus restrict the use of many broad-host-range plasmids in them as cloning vehicles. Antibiotic-sensitive mutants of Z. mobilis were isolated and used as hosts for the conjugal transfer of broad-host-range plasmids from Escherichia coli. Such antibiotic-sensitive strains can facilitate the application of broad-host-range plasmids to the study of Z. mobilis.
野生型运动发酵单胞菌表现出多种抗生素抗性，因此限制了许多广泛宿主范围质粒在其中作为克隆载体的使用。运动发酵单胞菌的抗生素敏感突变体被分离出来，并被用作大肠杆菌中广泛宿主范围质粒的接合转移的宿主。这种抗生素敏感菌株可以促进广泛宿主范围质粒在运动发酵单胞菌研究中的应用。
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