Eddy C K, Smith O H, Noel K D
Department of Biology, Marquette University, Milwaukee, Wisconsin 53233.
J Bacteriol. 1988 Jul;170(7):3158-63. doi: 10.1128/jb.170.7.3158-3163.1988.
A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. The trpF, trpB, and trpA genes were on one cosmid, while the trpD and trpC genes were on two separate cosmids. The organization of the Z. mobilis trp genes seems to be similar to the organization found in Rhizobium spp., Acinetobacter calcoaceticus, and Pseudomonas acidovorans. The trpF, trpB, and trpA genes appeared to be linked, but they were not closely associated with trpD or trpC genes.
构建了运动发酵单胞菌基因组DNA文库，该文库采用广宿主黏粒pLAFR1构建。利用辅助质粒pRK2013将该文库导入多种大肠杆菌和恶臭假单胞菌色氨酸突变体中。通过对色氨酸突变体的互补能力鉴定出5个运动发酵单胞菌色氨酸基因。色氨酸F、色氨酸B和色氨酸A基因位于一个黏粒上，而色氨酸D和色氨酸C基因位于两个独立的黏粒上。运动发酵单胞菌色氨酸基因的组织方式似乎与在根瘤菌属、醋酸钙不动杆菌和食酸假单胞菌中发现的组织方式相似。色氨酸F、色氨酸B和色氨酸A基因似乎是连锁的，但它们与色氨酸D或色氨酸C基因没有紧密关联。
