Horn Point Environmental Laboratories, Center for Environmental and Estuarine Studies, University of Maryland, Cambridge, Maryland 21613, and Bigelow Laboratory for Ocean Sciences, McKown Point, West Boothbay Harbor, Maine 04575.
Appl Environ Microbiol. 1986 Oct;52(4):935-8. doi: 10.1128/aem.52.4.935-938.1986.
Photosynthetic rates of phytoplankton were significantly lower after analysis by flow cytometry (FCM) than before. Exposure to the laser beam during the sorting process caused significant physiological damage. The cellular content of a radiolabel accumulated prior to FCM was not affected by FCM. Although it may not be possible to use FCM to preconcentrate cells for further physiological studies, samples may be incubated with stable or radioactive isotopes and then analyzed by FCM.
流式细胞仪（FCM）分析后，浮游植物的光合速率明显低于分析前。在分选过程中，激光束的照射会造成显著的生理损伤。FCM 之前放射性标记物积累的细胞内容物不受 FCM 的影响。尽管使用 FCM 对细胞进行预浓缩以进行进一步的生理研究可能不太可行，但可以用稳定或放射性同位素孵育样品，然后用 FCM 进行分析。
