流式细胞术分析对脆弱浮游植物形态和活力的影响。
Effects of flow cytometric analysis on morphology and viability of fragile phytoplankton.
机构信息
Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575.
出版信息
Appl Environ Microbiol. 1987 Nov;53(11):2677-9. doi: 10.1128/aem.53.11.2677-2679.1987.
Abstract
We assessed damage done to especially delicate marine phytoplankton cells by passage through a Coulter Epics V flow cytometer. The cells did not distort or lyse after exposure to fluidics or to laser light to 1,000 mW. The cells did sustain damage evidenced by temporary growth rate depressions. The four clones tested eventually resumed control growth rates after growth lags to 48 h.
摘要
我们评估了细胞通过库尔特 Epics V 流式细胞仪时受到的特别细微的海洋浮游植物细胞的损伤。细胞在暴露于液流或 1000mW 的激光下时没有变形或裂解。细胞确实受到了损伤，表现为暂时的生长率下降。经过 48 小时的生长滞后，测试的四个克隆最终恢复了对照生长率。
相似文献
1
Effects of flow cytometric analysis on morphology and viability of fragile phytoplankton.流式细胞术分析对脆弱浮游植物形态和活力的影响。
Appl Environ Microbiol. 1987 Nov;53(11):2677-9. doi: 10.1128/aem.53.11.2677-2679.1987.
2
Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes.有毒藻类衍生的多不饱和醛对培养的海洋浮游植物的生长抑制作用。
Aquat Toxicol. 2007 Dec 15;85(3):219-27. doi: 10.1016/j.aquatox.2007.09.006. Epub 2007 Sep 14.
3
Flow cytometric characterization of marine microbes.流式细胞术分析海洋微生物。
Methods. 2012 Jul;57(3):350-8. doi: 10.1016/j.ymeth.2012.07.001. Epub 2012 Jul 11.
4
Reticulocyte analysis using light microscopy and two different flow cytometric procedures.使用光学显微镜和两种不同的流式细胞术方法进行网织红细胞分析。
Pflugers Arch. 2000 Jan;440(Suppl 1):R185-R187. doi: 10.1007/s004240000058.
5
Photosynthetic characteristics of marine phytoplankton from pump-during-probe fluorometry of individual cells at sea.通过海上单细胞泵浦-探测荧光测定法对海洋浮游植物光合特性的研究
Cytometry. 1999 Sep 1;37(1):1-13. doi: 10.1002/(sici)1097-0320(19990901)37:1<1::aid-cyto1>3.0.co;2-c.
6
Reticulocyte analysis using light microscopy and two different flow cytometric procedures.使用光学显微镜和两种不同的流式细胞术程序进行网织红细胞分析。
Pflugers Arch. 2000;440(5 Suppl):R185-7.
7
A simple method to preserve oceanic phytoplankton for flow cytometric analyses.一种用于流式细胞术分析的保存海洋浮游植物的简单方法。
Cytometry. 1989 Sep;10(5):629-35. doi: 10.1002/cyto.990100519.
8
Discrimination of eukaryotic phytoplankton cell types from light scatter and autofluorescence properties measured by flow cytometry.通过流式细胞术测量的光散射和自发荧光特性对真核浮游植物细胞类型的区分。
Cytometry. 1989 Sep;10(5):636-43. doi: 10.1002/cyto.990100520.
9
Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces.用多环芳烃吸附在细胞表面的浮游细胞的流式细胞术检测。
Mar Pollut Bull. 2017 May 15;118(1-2):64-70. doi: 10.1016/j.marpolbul.2017.02.006. Epub 2017 Feb 17.
10
Underwater cytometer for in situ measurement of marine phytoplankton by a technique combining laser-induced fluorescence and laser Doppler velocimetry.通过激光诱导荧光和激光多普勒测速技术相结合的方法对海洋浮游植物进行原位测量的水下细胞计数器。
Opt Lett. 2005 May 15;30(10):1087-9. doi: 10.1364/ol.30.001087.
引用本文的文献
1
Viable cell sorting of dinoflagellates by multiparametric flow cytometry.利用多参数流式细胞术对甲藻进行活细胞分选。
Phycologia. 2009 Jul 1;48(4):249-257. doi: 10.2216/08-51.1.
2
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.异质微生物群体的流式细胞术和细胞分选：单细胞分析的重要性。
Microbiol Rev. 1996 Dec;60(4):641-96. doi: 10.1128/mr.60.4.641-696.1996.
3
Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting.通过流式细胞术和细胞分选技术从湖水和污水中快速、自动分离特定细菌。
Appl Environ Microbiol. 1993 Oct;59(10):3327-33. doi: 10.1128/aem.59.10.3327-3333.1993.
本文引用的文献
1
Effects of flow cytometric analysis and cell sorting on photosynthetic carbon uptake by phytoplankton in cultures and from natural populations.流式细胞分析和细胞分选对培养和自然种群浮游植物光合碳吸收的影响。
Appl Environ Microbiol. 1986 Oct;52(4):935-8. doi: 10.1128/aem.52.4.935-938.1986.
Zotero 插件