Natural Environment Research Council, Institute of Virology, Mansfield Road, Oxford OX1 3SR, United Kingdom, and Department of Biology, School of Sciences, Aristotelian University of Thessaloniki, Thessaloniki, Greece.
Appl Environ Microbiol. 1987 Jan;53(1):142-8. doi: 10.1128/aem.53.1.142-148.1987.
Representatives of several families of insect viruses were tested for growth and pathogenicity in the olive fruit fly, Dacus oleae Gmel. The viruses included nuclear polyhedrosis viruses, an iridovirus, two picornaviruses, and Trichoplusia ni small RNA virus (a member of the Nudaurelia beta family), in addition to two naturally occurring viruses of the olive fruit fly. Two viruses, one of the two picornaviruses (cricket paralysis virus [CrPV] and the iridovirus (type 21 from Heliothis armigera), were found to replicate in adult flies. Flies which were fed on a solution containing CrPV for 1 day demonstrated a high mortality with 50% dying within 5 days and nearly 80% dying within 12 days of being fed. The virus was transmissible from infected to noninfected flies by fecal contamination. The CrPV which replicated in the infected flies was demonstrated to be the same as input virus by infection of Drosophila melanogaster cells and examination of the expressed viral proteins, immunoprecipitation of the virus purified from flies, and electrophoretic analysis of the structural proteins.
几种昆虫病毒的代表株在油橄榄实蝇（Dacus oleae Gmel）中进行了生长和致病性测试。这些病毒包括核多角体病毒、虹彩病毒、两种微小 RNA 病毒（一种属于 Nudaurelia beta 科）和 Trichoplusia ni 小 RNA 病毒（一种属于 Nudaurelia beta 科），此外还有两种油橄榄实蝇的天然病毒。两种病毒，一种是两种微小 RNA 病毒之一（蟋蟀麻痹病毒[CrPV]和虹彩病毒（来自 Heliothis armigera 的 21 型）），在成年果蝇中复制。喂食含有 CrPV 的溶液 1 天的果蝇死亡率很高，有 50%的果蝇在 5 天内死亡，几乎 80%的果蝇在 12 天内死亡。该病毒可通过粪便污染从感染的果蝇传播给未感染的果蝇。通过感染果蝇细胞和检测表达的病毒蛋白、从果蝇中纯化病毒的免疫沉淀以及对结构蛋白的电泳分析，证明在感染的果蝇中复制的 CrPV 与输入病毒相同。
