Johnson K N, Christian P D
Botany and Zoology Department, Australian National University, Canberra, Australia.
Arch Virol. 1996;141(8):1509-22. doi: 10.1007/BF01718251.
Two new isolates of cricket paralysis virus, TAR and SIM, are described that were originally isolated from laboratory colonies of Drosophila melanogaster and Drosophila simulans respectively. Using a combination of biological, serological and molecular characters it was possible to distinguish the SIM isolate from all other isolates and it is thus described as a new strain; CrPVSIM. The TAR isolate however, was indistinguishable from the CrPV reference isolate CrPVVIC/GM/D2(2)/Gm/D2(2) (Teleogryllus commodus, Victoria, Australia, 1968). The molecular characters used in the present study were obtained by combining PCR and restriction endonuclease digestion of the amplified fragments. This work demonstrates that such molecular characters, when used in combination with others, provide a powerful set of taxonomic characters for classifying CrPV isolates and strains and assessing their genetic relatedness.
本文描述了蟋蟀麻痹病毒的两个新分离株，TAR和SIM，它们最初分别从黑腹果蝇和拟果蝇的实验室菌落中分离得到。通过结合生物学、血清学和分子特征，有可能将SIM分离株与所有其他分离株区分开来，因此将其描述为一个新的毒株；蟋蟀麻痹病毒SIM株（CrPVSIM）。然而，TAR分离株与蟋蟀麻痹病毒参考分离株CrPVVIC/GM/D2(2)/Gm/D2(2)（澳大利亚维多利亚州普通蝼蛄，1968年）无法区分。本研究中使用的分子特征是通过将聚合酶链反应（PCR）和扩增片段的限制性内切酶消化相结合获得的。这项工作表明，当这些分子特征与其他特征结合使用时，可为蟋蟀麻痹病毒分离株和毒株的分类以及评估它们的遗传相关性提供一套强大的分类学特征。
