Department of Microbiology, University of Groningen, 9751 NN Haren, The Netherlands.
Appl Environ Microbiol. 1987 Jan;53(1):149-55. doi: 10.1128/aem.53.1.149-155.1987.
Antisera against four different strains of Streptococcus cremoris were raised by injecting rabbits with washed suspensions of whole cells. These antisera interacted specifically with the corresponding strain in a mixture of up to nine different S. cremoris strains. The antisera could be used for analyzing the composition of mixed cultures containing these strains by immunofluorescence. Competition experiments were performed in batch and continuous cultures under amino acid limitation. A bacteriophage-sensitive variant of S. cremoris SK11 (SK1128) could be distinguished from a bacteriophage-resistant variant (SK1143) by the same immunofluorescence technique. The competition between the two variants and the stability of both variants in pure cultures were followed with the specific antibodies. Antibodies against the purified proteolytic system of S. cremoris Wg2 were used to determine the presence of proteases by immunofluorescence in several S. cremoris strains under different culture conditions. The described immunofluorescence methods can be used to analyze complex mixed starter cultures common in the dairy industry as the strains and variants present in these mixtures can be recognized microscopically.
用链球菌全细胞混悬液免疫兔子，制备了针对四种不同乳链球菌菌株的抗血清。这些抗血清在多达 9 种不同的乳链球菌菌株的混合物中与相应的菌株特异性相互作用。这些抗血清可用于通过免疫荧光分析含有这些菌株的混合培养物的组成。在氨基酸限制条件下的分批和连续培养中进行了竞争实验。通过相同的免疫荧光技术，可以将乳链球菌 SK11 的噬菌体敏感变体（SK1128）与噬菌体抗性变体（SK1143）区分开来。用特异性抗体跟踪两种变体之间的竞争以及两种变体在纯培养物中的稳定性。用乳链球菌 Wg2 的纯化蛋白水解系统的抗体通过免疫荧光在不同培养条件下的几种乳链球菌菌株中检测蛋白酶的存在。所描述的免疫荧光方法可用于分析乳制品工业中常见的复杂混合起始培养物，因为可以在显微镜下识别这些混合物中存在的菌株和变体。
