Environmental Engineering Laboratory, University of Aalborg, DK-9000 Aalborg, Denmark.
Appl Environ Microbiol. 1987 Jan;53(1):27-32. doi: 10.1128/aem.53.1.27-32.1987.
The sulfate kinetics in an anaerobic, sulfate-reducing biofilm were investigated with an annular biofilm reactor. Biofilm growth, sulfide production, and kinetic constants (K(m) and V(max)) for the bacterial sulfate uptake within the biofilm were determined. These parameters were used to model the biofilm kinetics, and the experimental results were in good agreement with the model predictions. Typical zero-order volume rate constants for sulfate reduction in a biofilm without substrate limitation ranged from 56 to 93 mumol of SO(4)-cm h at 20 degrees C. The temperature dependence (Q(10)) of sulfate reduction was equivalent to 3.4 at between 9 and 20 degrees C. The measured rates of sulfate reduction could explain the relatively high sulfide levels found in sewers and wastewater treatment systems. Furthermore, it has been shown that sulfate reduction in biofilms just a few hundred micrometers thick is limited by sulfate diffusion into biofilm at concentrations below 0.5 mM. This observation might, in some cases, be an explanation for the relatively poor capacity of the sulfate-reducing bacteria to compete with the methanogenic bacteria in anaerobic wastewater treatment in submerged filters.
采用环形生物膜反应器研究了厌氧硫酸盐还原生物膜中的硫酸盐动力学。确定了生物膜内细菌硫酸盐摄取的生物膜生长、硫化物生成和动力学常数（K(m)和 V(max)）。这些参数用于对生物膜动力学进行建模，实验结果与模型预测吻合良好。在无基质限制的生物膜中，典型的零级硫酸盐还原体积速率常数在 20°C 时范围为 56 至 93 mumol SO(4)-cm h。硫酸盐还原的温度依赖性（Q(10)）在 9 至 20°C 之间相当于 3.4。测量的硫酸盐还原速率可以解释在污水管道和废水处理系统中发现的相对较高的硫化物水平。此外，已经表明，在浓度低于 0.5 mM 的情况下，几百分之一毫米厚的生物膜中的硫酸盐还原受到硫酸盐向生物膜内扩散的限制。这种观察结果在某些情况下可能是硫酸盐还原细菌在淹没式过滤器中与产甲烷细菌竞争厌氧废水处理能力相对较差的原因之一。
