Laboratory of Microbial Ecology, State University of Ghent, Coupure L 653, B-9000 Ghent, Belgium.
Appl Environ Microbiol. 1986 Mar;51(3):572-9. doi: 10.1128/aem.51.3.572-579.1986.
The effect of different substrates and different levels of sulfate and sulfide on methane production relative to sulfate reduction in high-rate anaerobic digestion was evaluated. Reactors could be acclimated so that sulfate up to a concentration of 5 g of sulfate S per liter did not significantly affect methanogenesis. Higher levels gave inhibition because of salt toxicity. Sulfate reduction was optimal at a relatively low level of sulfate, i.e., 0.5 g of sulfate S per liter, but was also not significantly affected by higher levels. Both acetoclastic and hydrogenotrophic methane-producing bacteria adapted to much higher levels of free H(2)S than the values reported in the literature (50% inhibition occurred only at free H(2)S levels of more than 1,000 mg/liter). High levels of free H(2)S affected the sulfate-reducing bacteria only slightly. Formate and acetate supported the sulfate-reducing bacteria very poorly. In the high-rate reactors studied, intensive H(2)S formation occurred only when H(2) gas or an H(2) precursor such as ethanol was supplied.
评估了不同基质和不同浓度的硫酸盐和硫化物对高负荷厌氧消化中甲烷生成相对于硫酸盐还原的影响。可以使反应器适应环境，以至于每升 5 克硫酸盐 S 的硫酸盐浓度不会显著影响产甲烷作用。由于盐毒性，更高的浓度会产生抑制。硫酸盐还原在相对较低的硫酸盐水平（即每升 0.5 克硫酸盐 S）下达到最佳，但高水平的硫酸盐对其影响不大。产乙酸和氢营养型甲烷生成菌适应的游离 H 2 S 水平远高于文献中报道的值（只有在游离 H 2 S 水平超过 1000 毫克/升时才会出现 50%的抑制）。高水平的游离 H 2 S 对硫酸盐还原菌的影响很小。甲酸盐和乙酸盐对硫酸盐还原菌的支持作用很差。在所研究的高负荷反应器中，只有当供应氢气气体或乙醇等氢气前体时，才会发生强烈的 H 2 S 形成。
