Station de Pathologie Végétale et Phytobactériologie, Institut National de la Recherche Agronomique, Angers, F-49000 Beaucouzé, and Station d'Amélioration des Arbes Forestiers, Institut National de la Recherche Agronomique, Orléans, Ardon F-45160 Olivet, France.
Appl Environ Microbiol. 1987 Apr;53(4):655-9. doi: 10.1128/aem.53.4.655-659.1987.
This study focused on the natural crown gall infections occurring in a Leuce poplar nursery. Soil effects on crown gall frequency were detected, indicating that contamination was due to a resident Agrobacterium tumefaciens population, which was present before seedling plantation. The crown gall frequency on poplar progenies varied from 3 to 67%, indicating the feasibility of improvement in crown gall resistance. Of 129 tumor isolates, 128 were pathogenic. These isolates were of biotype 1 or 2. Biochemical, serological, and antibiotic resistance typing results concurred, indicating the presence of four biotype 1 and two biotype 2 resident subpopulations. No significant change was noticed in the relative proportions of subpopulations from one year to another. Pathogenic subpopulations both in vitro and in planta were susceptible to Kerr K84 (P. B. New and A. Kerr, J. Appl. Bacteriol. 90:172-179, 1972). In addition, no serological cross-reactions were found to occur between K84 and the pathogenic subpopulations.
本研究专注于杨树苗圃中自然发生的冠瘿病感染。土壤对冠瘿病发生率的影响被检测到，表明污染是由于定植的根癌农杆菌种群造成的，该种群在幼苗种植前就已存在。杨树苗后代的冠瘿病发生率从 3%到 67%不等，表明提高冠瘿病抗性是可行的。在 129 个肿瘤分离物中，有 128 个是病原性的。这些分离物属于生物型 1 或 2。生化、血清学和抗生素抗性分型结果一致，表明存在四个生物型 1 和两个生物型 2 定植亚群。从一年到另一年，亚群的相对比例没有明显变化。体外和体内的病原性亚群都容易受到 Kerr K84（P. B. New 和 A. Kerr，J. Appl. Bacteriol. 90:172-179, 1972）的影响。此外，在 K84 和病原性亚群之间没有发现血清学交叉反应。
