Station d'Amélioration des Arbres Forestiers, Institut National de la Recherche Agronomique, Ardon, F-45160 Olivet.
Appl Environ Microbiol. 1990 Nov;56(11):3537-45. doi: 10.1128/aem.56.11.3537-3545.1990.
Several Agrobacterium strains isolated from the same forest nursery from 1982 to 1988 were compared by serological, biochemical, and DNA-DNA hybridization methods. Similarities among strains belonging to biovar 2 were observed by indirect immunofluorescence, whereas biovar 1 strains showed serological heterogeneity. Electrophoretic analysis of bacterial envelope-associated proteins showed that few bands appeared in the strains belonging to biovar 1, whereas many proteins appeared in the case of biovar 2 strains. Chromosomal DNA was analyzed with six random C58 chromosomal fragments. None of the six probes hybridized to the DNA of the two biovar 2 strains. One of the probes gave the same hybridization pattern with all biovar 1 strains, whereas the other probes yielded different patterns. The vir regions were closely related in the different pathogenic strains. The T-DNA and replication regions were less conserved and showed some variations among the strains.
1982 年至 1988 年间，从同一森林苗圃中分离出的几种根癌农杆菌菌株，通过血清学、生物化学和 DNA-DNA 杂交方法进行了比较。间接免疫荧光观察到属于生物变种 2 的菌株之间存在相似性，而生物变种 1 菌株则表现出血清学异质性。细菌包膜相关蛋白的电泳分析表明，生物变种 1 菌株出现的条带很少，而生物变种 2 菌株则出现许多蛋白质。用 6 个随机 C58 染色体片段分析染色体 DNA。这 6 个探针都没有与两种生物变种 2 菌株的 DNA 杂交。其中一个探针与所有生物变种 1 菌株具有相同的杂交模式，而其他探针则产生不同的模式。不同致病菌株的 vir 区密切相关。T-DNA 和复制区的保守性较差，菌株之间存在一些变异。
