基于底物亲和性的果胶酸裂解酶从胡萝卜欧文氏菌中的快速纯化方法。
A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity.
机构信息
Agriculture Canada, Research Station, Vancouver, British Columbia, Canada, V6T 1X2.
出版信息
Appl Environ Microbiol. 1987 May;53(5):1189-91. doi: 10.1128/aem.53.5.1189-1191.1987.
Abstract
To purify pectate lyase produced by Erwinia carotovora subsp. carotovora, we used the supernatant from 48-h-old cultures grown in broth containing sodium polypectate and yeast extract. The supernatant was combined with the enzyme substrates sodium polypectate and polygalacturonic acid, which were then precipitated with CaCl(2). After the precipitate was washed, pectate lyase was eluted with 1.0 M NaCl.
摘要
为了纯化胡萝卜软腐果胶酶，我们使用了在含有聚半乳糖醛酸钠和酵母提取物的肉汤中培养 48 小时的细菌培养物的上清液。将上清液与酶底物聚半乳糖醛酸钠和多聚半乳糖酸混合，然后用氯化钙沉淀。沉淀洗涤后，用 1.0 M NaCl 洗脱果胶酶。
Zotero 插件