胡萝卜软腐欧文氏菌果胶酸裂解酶基因的克隆及其在大肠杆菌中的表达。
Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli.
作者信息
Lei S P, Lin H C, Heffernan L, Wilcox G
出版信息
Gene. 1985;35(1-2):63-70. doi: 10.1016/0378-1119(85)90158-1.
A hybrid cosmid coding for pectate lyase (PL) activity was identified from an Erwinia carotovora genomic library by an immunological screening method. A 7-kb DNA fragment was identified which codes for three proteins identical in size to proteins with PL activity purified from E. carotovora culture supernatants. The three proteins had apparent Mrs of 41, 44 and 44 X 10(3) as estimated by SDS-PAGE. None of the PLs were exported from Escherichia coli strain HB101 but all were found in the periplasmic space. Plant tissue was macerated by the PLs made in E. coli.
通过免疫筛选法从胡萝卜软腐欧文氏菌基因组文库中鉴定出一个编码果胶酸裂解酶（PL）活性的杂种黏粒。鉴定出一个7kb的DNA片段，它编码三种蛋白质，其大小与从胡萝卜软腐欧文氏菌培养上清液中纯化的具有PL活性的蛋白质相同。通过SDS-PAGE估计，这三种蛋白质的表观分子量分别为41×10³、44×10³和44×10³。这些PL均未从大肠杆菌HB101菌株中分泌出来，但都存在于周质空间中。大肠杆菌中产生的PL可使植物组织软化。
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