Battelle-Kettering Laboratory, Yellow Springs, Ohio 45387.
Appl Environ Microbiol. 1987 Jul;53(7):1397-405. doi: 10.1128/aem.53.7.1397-1405.1987.
Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strain 2-79, and the pilin subunit was found to have a molecular weight of about 34,000. Strain 2-79 produced two colony types on Luria agar, nonmucoidal and mucoidal. Cells in LNM cultures of the nonmucoidal colony type were highly piliated, and cells from the mucoidal type were nearly devoid of pili. The presence of pili on nonmucoidal isolate 2-79 was quantitatively correlated with hydrophobic attachment to polystyrene, hemagglutination, and attachment to corn roots.
荧光假单胞菌分离株 13525 和 2-79 在 LB 培养液和低营养培养基(LNM)中生长。在 LB 培养液中很少产生菌毛状纤维,但在 LNM 中大量产生。在 LNM 中,菌毛呈周生分布,直径为 3 至 8nm。从菌株 2-79 中纯化出菌毛,发现菌毛亚基的分子量约为 34000。在 LB 琼脂上,2-79 株产生两种菌落类型,非粘液型和粘液型。非粘液型菌落类型的 LNM 培养物中的细胞高度菌毛化,而粘液型的细胞几乎没有菌毛。非粘液型分离株 2-79 菌毛的存在与聚苯乙烯的疏水性附着、血凝和玉米根附着呈定量相关。