Jackwood M W, Saif Y M
Avian Dis. 1987 Apr-Jun;31(2):277-86.
Electron microscopy revealed pili on all isolates of Bordetella avium and B. avium-like bacteria examined. Trypticase soy broth (TSB) and 2% peptone agar were the best media for promoting pilus expression. Cultures grown at 37 or 42 C had similar pilus production, whereas cultures grown at 18 C produced few or no pili. Pilus expression of the Art Vax strain was best when that strain was grown in TSB, but the strain yielded fewer pili than B. avium and B. avium-like isolates grown under the same cultural conditions. B. avium pili had a diameter of 2.0 nm, ranged in length from 370 nm to 1500 nm, and had a protein subunit molecular mass of about 13,100 daltons. Purified pili from B. avium did not hemagglutinate guinea pig erythrocytes, and a 1:20 dilution of hyperimmune antisera against B. avium pili did not block the hemagglutinating activity of whole-cell preparations of B. avium. In the indirect immunofluorescence test, B. avium isolates and the Art Vax strain adhered to the tracheal explants of turkeys, but B. avium-like isolates did not. Purified pili from B. avium adhered to the surface of the mucosal lining of the tracheal explants, and hyperimmune antisera against B. avium pili blocked the in vitro adherence of whole-cell preparations of B. avium. It was concluded that pili of B. avium are involved in the in vitro attachment of that bacterium to the mucosal surface of turkey tracheal explants.
电子显微镜检查显示，在所检测的所有禽博德特氏菌和类禽博德特氏菌分离株上均发现了菌毛。胰蛋白胨大豆肉汤（TSB）和2%蛋白胨琼脂是促进菌毛表达的最佳培养基。在37℃或42℃培养的菌落在菌毛产生方面相似，而在18℃培养的菌落产生的菌毛很少或不产生菌毛。当Art Vax菌株在TSB中生长时，其菌毛表达最佳，但该菌株产生的菌毛比在相同培养条件下生长的禽博德特氏菌和类禽博德特氏菌分离株少。禽博德特氏菌菌毛直径为2.0 nm，长度在370 nm至1500 nm之间，并具有约13,100道尔顿的蛋白质亚基分子量。从禽博德特氏菌中纯化的菌毛不会使豚鼠红细胞发生血凝，针对禽博德特氏菌菌毛的超免疫抗血清的1:20稀释液不会阻断禽博德特氏菌全细胞制剂的血凝活性。在间接免疫荧光试验中，禽博德特氏菌分离株和Art Vax菌株黏附于火鸡气管外植体，但类禽博德特氏菌分离株则不黏附。从禽博德特氏菌中纯化的菌毛黏附于气管外植体黏膜内衬表面，针对禽博德特氏菌菌毛的超免疫抗血清可阻断禽博德特氏菌全细胞制剂的体外黏附。得出的结论是，禽博德特氏菌的菌毛参与了该细菌在体外与火鸡气管外植体黏膜表面的附着。
