Rebers P A, Jensen A E, Laird G A
U.S. Department of Agriculture, National Animal Disease Center, Ames, Iowa 50010.
Avian Dis. 1988 Apr-Jun;32(2):313-8.
The avian strain P-1059 of Pasteurella multocida was grown on blood agar (BA), on dextrose-starch agar (DSA), or in Heddleston's hydrogen sulfide test broth. Cells were examined for the presence of pili using electron microscopy after staining with phosphotungstic acid, and they were examined for capsule after ruthenium red staining. Pili were found on the capsulated iridescent type, P-1059I, and on two non-capsulated variants, the blue, P-1059B, and the gray, P-1059G. Many cells grown on BA were heavily piliated. In contrast, fewer cells grown on DSA had pili, and piliation was only slight to moderate. The P-1059I, P-1059B, and P-1059G produced pellicles when grown on broth medium. Pili were found on the circumference of the cells grown on either agar or broth medium. Occasionally a pilus connecting two cells was seen on cells cultured in broth. Cultivation of the P-1059I on DSA containing the iron-chelating agent alpha,alpha'-bipyridyl produced a non-capsulated blue variant. The non-capsulated variant reverted to P-1059I when grown on BA but did not revert when grown on DSA.
多杀性巴氏杆菌禽源菌株P - 1059在血琼脂（BA）、葡萄糖 - 淀粉琼脂（DSA）或赫德尔斯顿硫化氢试验肉汤中培养。用磷钨酸染色后，通过电子显微镜检查细胞是否存在菌毛，并用钌红染色检查细胞是否有荚膜。在有荚膜的虹彩型P - 1059I以及两个无荚膜变体，蓝色的P - 1059B和灰色的P - 1059G上发现了菌毛。在BA上生长的许多细胞有大量菌毛。相比之下，在DSA上生长的细胞有菌毛的较少，且菌毛形成仅为轻度到中度。P - 1059I、P - 1059B和P - 1059G在肉汤培养基中生长时会产生菌膜。在琼脂或肉汤培养基上生长的细胞的周边发现了菌毛。偶尔在肉汤中培养的细胞上会看到连接两个细胞的菌毛。在含有铁螯合剂α，α'-联吡啶的DSA上培养P - 1059I会产生一种无荚膜的蓝色变体。这种无荚膜变体在BA上生长时会回复为P - 1059I，但在DSA上生长时不会回复。
