Environmental and Occupational Health Sciences, School of Public Health, University of California, Los Angeles, California 90024.
Appl Environ Microbiol. 1987 Jul;53(7):1589-92. doi: 10.1128/aem.53.7.1589-1592.1987.
Enrichment cultures were used to determine the conditions promoting fastest methanogenic propionate degradation and growth by adapting the cultures to various physical and chemical conditions and measuring the specific growth rate. We found that the fastest growth of propionate oxidizers occurred at pH 6.8 to 8.5 and 32 to 45 degrees C. Acetate-degrading populations showed narrower optima for fastest growth (pH 6.8 to 7.2 and 37 to 43 degrees C). Enrichment cultures grew as well in minimal medium as in complex medium, although individual microbial populations appeared to require growth factors which could be met by cross-feeding.
采用富集培养的方法,通过适应各种物理化学条件并测量特定生长速率,来确定促进产甲烷丙酸最快降解和生长的条件。我们发现,产丙酸氧化菌的最快生长发生在 pH 值为 6.8 到 8.5 和 32 到 45 摄氏度。乙酸降解种群的最快生长最适范围较窄(pH 值为 6.8 到 7.2 和 37 到 43 摄氏度)。富集培养在基础培养基和复杂培养基中都能生长,尽管单个微生物种群似乎需要生长因子,可以通过交叉喂养来满足。