嗜热纤维单胞菌对纤维二糖的优先利用。
Preferential Utilization of Cellobiose by Thermomonospora curvata.
机构信息
Department of Microbiology, Clemson University, Clemson, South Carolina 29631.
出版信息
Appl Environ Microbiol. 1987 Aug;53(8):1743-7. doi: 10.1128/aem.53.8.1743-1747.1987.
Abstract
Thermomonospora curvata was cultivated on mineral salts medium containing glucose and cellobiose under conditions that increasingly favored the uptake of glucose. In each case cellobiose was utilized in preference to glucose and induced beta-glucosidase and endoglucanase activity. [C]glucose metabolism studies indicated that cellobiose was not cleaved by extracellular beta-glucosidase and transported as glucose. No evidence of cellobiose phosphorylase or a cellobiose-specific phosphoenolpyruvate-phosphotransferase system was observed.
摘要
嗜热链霉菌在含有葡萄糖和纤维二糖的无机盐培养基中培养，条件逐渐有利于葡萄糖的吸收。在每种情况下，纤维二糖都优先于葡萄糖被利用，并诱导β-葡萄糖苷酶和内切葡聚糖酶活性。[14C]葡萄糖代谢研究表明，纤维二糖不能被细胞外β-葡萄糖苷酶裂解，而是作为葡萄糖被运输。没有观察到纤维二糖磷酸化酶或纤维二糖特异性磷酸烯醇丙酮酸磷酸转移酶系统的证据。
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