Nitrogen Fixation and Soybean Genetics Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, and Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822.
Appl Environ Microbiol. 1987 Aug;53(8):1785-9. doi: 10.1128/aem.53.8.1785-1789.1987.
Several isolates of Rhizobium fredii were examined for their serological relatedness to each other, to Bradyrhizobium japonicum, and to other fast- and slow-growing rhizobia. Immunofluorescence, agglutination, and immunodiffusion analyses indicated that R. fredii contains at least three separate somatic serogroups, USDA 192, USDA 194, and USDA 205. There was no cross-reaction between any of the R. fredii isolates and 13 of the 14 B. japonicum somatic serogroups tested. Cross-reactions were obtained with antisera from R. fredii and serogroup 122 of B. japonicum, Rhizobium meliloti, and several fast-growing Rhizobium spp. for Leucaena, Sesbania, and Lablab species. The serological relationship between R. fredii and R. meliloti was examined in more detail, and of 23 R. meliloti strains examined, 8 shared somatic antigens with the type strains from all three R. fredii serogroups. The serological relatedness of R. fredii to B. japonicum and R. meliloti appears to be unique since the strains are known to be biochemically and genetically diverse.
对多个根瘤菌弗雷氏菌株进行了血清学相关性研究，以确定它们彼此之间、与慢生根瘤菌和其他快速生长和慢速生长根瘤菌之间的血清学关系。免疫荧光、凝集和免疫扩散分析表明，根瘤菌弗雷氏菌至少包含三个独立的菌体血清群，USDA192、USDA194 和 USDA205。在测试的 14 个慢生根瘤菌血清群中，没有任何一个根瘤菌弗雷氏菌分离株与 13 个发生交叉反应。与根瘤菌弗雷氏菌和血清群 122 的抗血清发生交叉反应，与根瘤菌 meliloti 和几种快速生长的根瘤菌属对银合欢、田菁和豇豆属的物种。详细研究了根瘤菌弗雷氏菌和根瘤菌 meliloti 之间的血清学关系，在 23 株根瘤菌 meliloti 菌株中，有 8 株与来自所有三个根瘤菌弗雷氏菌血清群的模式株具有相同的菌体抗原。根瘤菌弗雷氏菌与慢生根瘤菌和根瘤菌 meliloti 的血清学关系似乎是独特的，因为这些菌株在生化和遗传上都是多样化的。
