蛋白酶对乳酸链球菌原生质体转染和转化的影响。
Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts.
机构信息
Department of Nutrition and Food Sciences, Utah State University, Logan, Utah 84322-8700.
出版信息
Appl Environ Microbiol. 1987 Oct;53(10):2583-7. doi: 10.1128/aem.53.10.2583-2587.1987.
Abstract
With both chymotrypsin and mutanolysin used to form protoplasts, consistent transformation frequencies of 10 to 10 transformants and transfectants per mug of DNA were achieved. The procedure was used to transform protoplasts of Streptococcus cremoris CS224 at low frequency (5 transformants per mug of DNA).
摘要
用糜蛋白酶和溶菌酶两种酶都可制成原生质体，每毫克 DNA 可获得 10 到 100 个转化子和转染子的一致转化频率。该程序用于低频（每毫克 DNA 5 个转化子）转化乳球菌 CS224 的原生质体。
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