Anderson D G, McKay L L
Appl Environ Microbiol. 1983 Sep;46(3):549-52. doi: 10.1128/aem.46.3.549-552.1983.
A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.
本文描述了一种从乳酸链球菌中快速分离大于30兆道尔顿质粒DNA的方法。该方案可用于制备规模，以分离限制酶切分析、克隆或转化实验所需的足够量的质粒DNA。缩小规模的方案对于快速筛选链球菌菌株的质粒含量非常有用。通过这种方法，观察到了以前未检测到的大质粒。
