Becton Dickinson Biosciences, Advanced Cytometry Group, 12730 28th Ave NE, Seattle, WA 98125, USA.
Methods. 2012 Jul;57(3):350-8. doi: 10.1016/j.ymeth.2012.07.001. Epub 2012 Jul 11.
The analysis of marine phytoplankton using flow cytometry has enabled the discovery of new taxa and has contributed new understanding to the dynamics and ecological contributions of phytoplankton to the global carbon cycle. Marine phytoplankton are uniquely suited to analysis by flow cytometry because of their size, pigment content, and ability to remain in suspension. Cytometric analysis of marine populations is not without challenges. Phytoplankton communities span a broad range of sizes. The smallest microbes are a few tenths of a micron, while the largest are a few tenths of a millimeter. The improvement of cytometric measurements of scattered laser light allows one to investigate marine microbes whose sizes span several orders of magnitude. To effectively leverage the advantages that marine microbes possess, cytometers have to be carefully engineered for marine use.
利用流式细胞术分析海洋浮游植物使人们发现了新的分类群，并加深了对浮游植物在全球碳循环中的动态和生态贡献的理解。由于海洋浮游植物的大小、色素含量和悬浮能力，它们非常适合通过流式细胞术进行分析。流式细胞术分析海洋种群并非没有挑战。浮游植物群落的大小跨度很广。最小的微生物只有几十分之一微米，而最大的微生物则有几十分之一毫米。对散射激光光的细胞测量的改进使得人们能够研究大小跨越几个数量级的海洋微生物。为了有效地利用海洋微生物所具有的优势，必须为海洋应用精心设计细胞仪。
