温度和 pH 值对美洲棉铃虫多核型多角体病毒存活的影响。

Effects of Temperature and pH on Survival of Free Nuclear Polyhedrosis Virus of Autographa californica.

机构信息

Environmental Research Laboratory, U.S. Environmental Protection Agency, 200 S.W. 35th Street, Corvallis, Oregon 97333.

出版信息

Appl Environ Microbiol. 1987 Dec;53(12):2771-3. doi: 10.1128/aem.53.12.2771-2773.1987.

DOI:10.1128/aem.53.12.2771-2773.1987

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204196/

Abstract

The effects of temperature and low pH on replication and survival of nonoccluded Autographa californica nuclear polyhedrosis virus were investigated. No virus replication or formation of polynuclear inclusion bodies occurred at 37 degrees C. The virus was immediately inactivated upon exposure to pH 2.0 and was inactivated within 1 h at pH 4.0. The virus titer slowly declined, a 3-orders of magnitude reduction in virus titer, at pH 5.0 during a 4-h exposure. Virus survival at pH 6.0 was equal to that of the control in cell culture medium 199 MK (pH 7.12).

摘要

研究了温度和低 pH 值对非包埋的美洲棉铃虫核多角体病毒复制和存活的影响。在 37°C 时，没有病毒复制或多核包涵体的形成。病毒在接触 pH 值 2.0 时立即失活，在 pH 值 4.0 时在 1 小时内失活。在 pH 值 5.0 下暴露 4 小时，病毒滴度缓慢下降，病毒滴度降低 3 个数量级。在 pH 值 6.0 下，病毒在细胞培养液 199 MK（pH 值 7.12）中的存活与对照相同。

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