Wood H A, Johnston L B, Burand J P
Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, New York 14853, USA.
Virology. 1982 Jun;119(2):245-54. doi: 10.1016/0042-6822(82)90085-x.
Replication of the Autographa californica nuclear polyhedrosis virus was studied in Trichoplusia ni (TN-368) tissue culture cells under cell density conditions which approximated a confluent monolayer (5.7 x 10(5) cells/cm(2) growth surface). These high-density cultures were not refractory to infection but the synthesis of both the occluded and nonoccluded forms of the virus were inhibited greater than 98% when compared to inoculation of low cell density cultures (1.4 x 10(5) cells/cm(2)). The inhibition phenomenon was not due to depletion of a medium component or a diffusable cell-associated factor. The inhibition was reversible and required cell-to-cell contact. Viral-induced protein synthesis studies using 35S-labeled methionine incorporation indicated an 18-hr delay in the synthesis of one of the four early proteins under high cell density conditions. DNA:DNA dot hybridization and 3H-labeled thymidine incorporation studies indicated that cell-to-cell contact reversibly inhibited both cellular and viral DNA synthesis.
在近似汇合单层（生长表面为5.7×10⁵个细胞/cm²）的细胞密度条件下，研究了苜蓿银纹夜蛾核型多角体病毒在粉纹夜蛾（TN - 368）组织培养细胞中的复制情况。这些高密度培养物对感染并非难治，但与低细胞密度培养物（1.4×10⁵个细胞/cm²）接种相比，病毒的包涵体形式和非包涵体形式的合成均受到大于98%的抑制。抑制现象并非由于培养基成分的耗尽或可扩散的细胞相关因子所致。这种抑制是可逆的，且需要细胞间接触。使用³⁵S标记的甲硫氨酸掺入进行的病毒诱导蛋白合成研究表明，在高细胞密度条件下，四种早期蛋白之一的合成延迟了18小时。DNA:DNA点杂交和³H标记的胸腺嘧啶掺入研究表明，细胞间接触可逆地抑制了细胞和病毒的DNA合成。
