Wood H A
Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York 14854, USA.
Virology. 1980 Apr 15;102(1):21-7. doi: 10.1016/0042-6822(80)90066-5.
The multiplication of Autographa californica nuclear polyhedrosis virus was studied in Trichoplusia ni and Spodoptera frugiperda insect cell lines. Using pulse labeling, 18 viral-induced proteins were identified by polyacrylamide gel electrophoresis. Based on infectivity curves and protein synthesis studies, replication could be divided into four phases. During the early phase of infection, four viral-induced proteins with molecular weights of 45, 35, 34, and 31 thousand daltons could be detected. Maturation of the nonoccluded form of the virus and synthesis of 10 viral-induced proteins with molecular weights of 67, 61, 49, 41, 39, 26, 24, 22, 21, and 16 thousand daltons were first detected during the intermediate phase of replication, 10 to 16 hr postinoculation (p.i.). During the transitional phase from nonoccluded to occluded virus formation (16 to 24 hr p.i.), four additional viral-induced proteins could be detected with molecular weights of 33, 27, 15.7, and 15 thousand daltons. No additional viral-induced proteins were detected during the late phase of occluded virus formation. Post-translational cleavage was not detected in pulse-chase experiments. The addition of varying concentrations of NaCl did not selectively inhibit cellular protein synthesis.
在粉纹夜蛾和草地贪夜蛾昆虫细胞系中研究了苜蓿银纹夜蛾核型多角体病毒的增殖情况。采用脉冲标记法，通过聚丙烯酰胺凝胶电泳鉴定出18种病毒诱导蛋白。根据感染性曲线和蛋白质合成研究，复制可分为四个阶段。在感染早期，可检测到四种分子量分别为45、35、34和31千道尔顿的病毒诱导蛋白。在复制中期，接种后10至16小时，首次检测到病毒非包涵体形式的成熟以及10种分子量分别为67、61、49、41、39、26、24、22、21和16千道尔顿的病毒诱导蛋白的合成。在从非包涵体病毒形成到包涵体病毒形成的过渡阶段（接种后16至24小时），可检测到另外四种分子量分别为33、27、15.7和15千道尔顿的病毒诱导蛋白。在包涵体病毒形成后期未检测到其他病毒诱导蛋白。在脉冲追踪实验中未检测到翻译后切割。添加不同浓度的氯化钠并未选择性抑制细胞蛋白质合成。
