Département de microbiologie, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec J1N 4N4, Canada.
Appl Environ Microbiol. 1988 Jun;54(6):1550-6. doi: 10.1128/aem.54.6.1550-1556.1988.
We have screened a genomic library of Trametes versicolor for genes whose expression is associated with nitrogen starvation, which has been shown to induce ligninolytic activity. Using two different approaches based on differential expression, we isolated 29 clones. These were shown by restriction mapping and cross-hybridization to code for 11 distinct differentially expressed genes. Northern analysis of the kinetics of expression of these genes revealed that at least four of them have kinetics of induction that parallel kinetics of induction of ligninolytic activity.
我们已经从糙皮侧耳（Trametes versicolor）的基因组文库中筛选出了与氮饥饿相关表达的基因，氮饥饿已被证明能诱导木质素降解活性。使用两种基于差异表达的不同方法，我们分离出了 29 个克隆。通过限制图谱和交叉杂交显示，这些克隆编码 11 个不同的差异表达基因。对这些基因表达动力学的 northern 分析表明，至少有 4 个基因的诱导动力学与木质素降解活性的诱导动力学平行。
